Fruit flies are one of the easiest feeder insects to care for. Each 32 oz fruit fly culture includes all the food and water the flies will need for 4 to 8 weeks. Freshly Started Hydei Cultures will start to produce flies you can feed to your animals in 17-21 days. Freshly Started Melanogaster Cultures will start producing flies in 10-14 days. Each culture has the potential to produce 1000s of flies, but there are a few things that can slow down or stop production all together. The key to great production from your cultures is the environment you keep your cultures in.